Exponential megapriming PCR (EMP) cloning--seamless DNA insertion into any target plasmid without sequence constraints.

We present a fast, reliable and inexpensive restriction-free cloning method for seamless DNA insertion Oven into any plasmid without sequence limitation.Exponential megapriming PCR (EMP) cloning requires two consecutive PCR steps and can be carried out in one day.We show that EMP cloning has a higher efficiency than restriction-free (RF) cloning, e

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Letter of Editor-in-Chief

Dear Colleagues and students As Editor-in-Chief of Journal Course a pied - Enfant - Chaussures - Ligth trainer of Cardio-Thoracic Medicine, I would like to dedicate my especial respect and appreciation for your kind regards and consideration to our journal.Fortunately, it is our pleasure to announce that Journal of Cardio-Thoracic Medicine is now i

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Designation and psychometric properties of the Short Form Postpartum Quality of Life Questionnaire (SF-PQOL): an application of multidimensional item response theory and genetic algorithm

Background: Utilizing multidimensional item response theory (MIRT) and genetic algorithm(GA) we aimed to design and test the psychometric properties of the short form Postpartum Quality of Heart Shaped Acrylic Plaque Life Questionnaire (PQOL).Methods: In this methodological study, 500 women aged 18 to 42 were enrolled through multistage random samp

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